Applications: Suitable for use in FLISA and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by researcher. Label: Aminomethylcoumarin (AMCA) C16H14N2O9S (Molecular Weight 410 daltons) Absorption Wavelength: 353 nm Emission Wavelength: 440 nm Fluorochrome/Protein Ratio: 8.2 moles AMCA per mole of Streptavidin A353/A280 Ratio: 0.68 Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
Formulierung:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide.
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