Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react non-specifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level. Negligible non-specific binding to non-biotinylated macromolecules, giving very low backgrounds. Applications: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen/paraffin): 1:500 ELISA: 1:1000-1:10,000 Optimal dilutions to be determined by researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified
Formulierung:
Supplied as a liquid in pBS, 0.01% thimerosal. Labeld with Horseradish peroxidase (HRP).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
