Recombinant mouse SDF-1 alpha, expressed in E. coli.
SDF-1 alpha, otherwise known as CXCL12a, a stromal cell derived CXC chemokine which, together with SDF-1 beta, arises from alternative splicing of the SDF-1 gene, and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons. SDF-1 binds with high-affinity to the G protein-coupled receptor CXCR4 (fusin), and acts as an inhibitor of the CXCR4-mediated entry of HIV-1 virus into target T-cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-2ug/ml Western Blotting: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
