SDF-1 alpha, otherwise known as CXCL12a, is a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF-1 gene. The two isoforms, SDF-1 alpha and SDF-1 beta, share an identical amino acid sequence, except for an additional four residues in the C-terminal region of the beta isoform. SDF-1 binds with high-affinity to the G protein-coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in hematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF-1 acts as an inhibitor for the CXCR4-mediated entry of HIV-1 virus into target T-cells, and the SDF-1-CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells. Applications: Suitable for use in ELISA, Functional Assays, and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.15-0.3ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 2-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
