SDF-1 alpha, otherwise known as CXCL12a, is a stromal cell derived CXC chemokine which, together with SDF-1 beta, arises from alternative splicing of the SDF-1 gene, and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons. SDF-1 binds with high-affinity to the G protein-coupled receptor CXCR4 (fusin), and acts as an inhibitor of the CXCR4-mediated entry of HIV-1 virus into target T-cells. Source: E. coli Applications: Suitable for use in Functional Assays. Other applications not tested. Biological Activity: Determined by its ability to chemoattract human peripheral blood monocytes, using a concentration of 50-100ng/ml. Molecular Weight: 7.9kD (68 amino acid residue homodimer) Amino Acid Sequence: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK Storage and Stability: Lyophilized and reconstituted products may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
