Recombinant protein corresponding to mouse SDF-1b expressed in E. coli
SDF-1b (stromal cell-derived factor-b), also known as pre-B-cell growth-stimulating factor (PBSF), is a 74-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis in mice. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry. Applications: Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry: 1:100 Immunohistochemistry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by Protein A affinity chromatography
Formulierung:
Supplied as a liquid, 0.05% sodium azide
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