STYX (Serine/Threonine/Tyrosine-interacting Protein, Protein Tyrosine Phosphatase-like Protein) (FITC), Rabbit
Artikelnummer:
USB-S7985-50-FITC
Hersteller Artikelnummer:
S7985-50-FITC
Alternativnummer:
USB-S7985-50-FITC-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
WB
Immunogen:
Full length protein corresponding to aa1-223 of human STYX. (NP_660294.1)
STYX belongs to the protein-tyrosine phosphatase family, Non-receptor class subfamily. It contains one tyrosine-protein phosphatase domain and is a probable pseudophosphatase. It has a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and may be protect them from phosphatases. STYX seems to play a role in spermiogenesis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000, Band is observed at ~25.5kD using STYX-transfected 293T lysate Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
