Sulfotransferase 1A3, Cytosolic, Recombinant, Human (SULT1A3)
Artikelnummer:
USB-S8022-03B
Hersteller Artikelnummer:
S8022-03B
Alternativnummer:
USB-S8022-03B-20
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Cytosolic sulfotransferases are a family of Phase II drugmetabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (13). They have important roles in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are widely distributed throughout the body and serve to inactivate and increase watersolubility of xenobiotics and therapeutic drugs. They are distinct from Golgi resident sulfotransferases by lacking N terminal signalanchor domains and residing only in the cytoplasm. SULT1A3 catalyzes the sulfation of phenolic monoamines, such as dopamine, norepinephrine and serotonin, and phenolic and catecholic drugs (4, 5). SULT1A3 is also called catecholamine sulfotransferase. Recombinant enzyme corresponding to Glu2-Leu295 with a N-terminal Met and 6 His tag of human Sulfotransferase 1A3 expressed in E. coli. Swiss/UniProt Accession: P50224 N-terminal Sequence Analysis: Met Predicted Molecular Mass: 35kD SDS-PAGE: 34kD, reducing conditions Activity: Measured by its ability to transfer sulfate from PAPS to 1-Napthol. Specific Activity: 15pmoles/min/ug, as measured under the described conditions (see Protocol). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Stability & Storage: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
