TACE, CT, Recombinant, Rat, Control (TNF-alpha Converting Enzyme)

Produktübersicht

• Artikelname: TACE, CT, Recombinant, Rat, Control (TNF-alpha Converting Enzyme)
• Artikelnummer: USB-T0500-11
• Hersteller Artikelnummer: T0500-11
• Alternativnummer: USB-T0500-11-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA, WB

Produktbeschreibung

Beta-amyloid (Ab) deposition in the brain is the hallmark of Alzheimers Disease (AD). To initiate Ab formation, beta-secretase cleaves APP at the N-terminus of Ab to release APPsb (~100kD soluble NT-fragment), and C99, a 12-kD CT membrane fragment. Alternatively, alpha-secretase cleaves within the Ab to prevent the formation of Ab. Cleavage by a-secretase produces a soluble N-terminal fragment, APPsa, and a 10-kD membrane C-terminal fragment, C83. Both C99 and C83 can be further cleaved by gamma-secretase releasing Ab and a nonpathogenic p3 peptide, respectively. Recently TACE, a member of the ADAM family (A Disintegrin And Metalloprotease family) protease has been shown to play a central role in a regulated cleavage of human APP. Inhibition of TACE affects both APP secretion and Ab formation in cultured cells (1). Membrane-bound TNF-a, like APP, is transmembrane protein that can undergo TACE-mediated proteolysis to release the extracellular domain as soluble TNF-a. TACE contain an autoinhibitory domain that must be removed for activity, a proteolytic domain, a disintegrin domain, a cysteine-rich domain, and a Transmembrane domain. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Purified
• Formulierung: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.