TAGLN2, also known transgelin 2, is one of the earliest markers of differentiated smooth muscle. This protein contains a calponin like repeat and a calponin-homology (CH) domain. It is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. It also binds actin, causing actin fibers to gel within minutes of binding. Binding of transgelin to actin occurs at a ratio of 1:6 actin monomers. Source: Recombinant corresponding to human TAGLN2, fused to His-tag at N-terminus, expressed in E. coli. Molecular Weight: ~23.4kD Sequences of Amino Acids: MGSSHHHHHH SSGLVPRGSH MEVQQKIEKQ YDADLEQILI QWITTQCRKD VGRPQPGREN FQNWLKDGTV LCELINALYP EGQAPVKKIQ ASTMAFKQME QISQFLQAAE RYGINTTDIF QTVDLWEGKN MACVQRTLMN LGGLAVARDD GLFSGDPNWF PKKSKENPRN FSDNQLQEGK NVIGLQMGTN RGASQAGMTG YGMPRQIL Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
