TaqI

Produktübersicht

• Artikelname: TaqI
• Artikelnummer: USB-T1017
• Hersteller Artikelnummer: T1017
• Alternativnummer: USB-T1017-3000,USB-T1017-5X3000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-T C G A-3 3-A G C T-5 Source: Thermus aquaticus YT-1 Supplied wtih: R1625-80: Restriction Enzyme Buffer for TaqI, 10X: Dilute to 1X for use. 1X buffer composition 10mM Tris-HCl pH 8.0, 5mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Use as 1x. R1625 - Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA Diluent Buffer: 10mM Tris-HCl pH 7.4,100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl pH 7.5, 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol. Compatible Ends: Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, Ssil, XmiI Unit Definition: One unit is defined as the amount of TaqI to digest 1ug of lambda DNA dam- in 1 hour at 65C in 50ul of assay buffer. Thermal Inactivation: Enzyme is not inactivated by incubation at 80C for 20min. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA dam-x 16 hours) with TaqI. Ligation/Recleavage Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of TaqI for 4 hours. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.