Tetanus Toxin is polypeptide composed of two chains [A chain (55kD) and B chain (105kD)]. The toxin is carried to CNS via bloodstream. Tetanus Toxin binds at neuromuscular synapses via ganglioside receptors (sialic acid) via B-chain. The toxin is then internalized via receptor-mediated endocytosis. The A chain is thought to be the toxic subunit by analogy to diptheria and cholera toxin. It has no known effect on any cellular enzyme systems. The action of the holotoxin (A + B chains) is to interfer with synaptic transmission by preferentially inhibiting the release of inhibitory neurotransmitters such as glycine and GABA from inhibitory neurons This inhibition causes rigid muscle contraction-spastic paralysis. Tetanus toxin is an exotoxin. These toxons are secreted by a bacterial cell into surrounding fluids. Exotoxins are produced by both Gram-negative and Gram-positive bacteria. Most exotoxins are readily destroyed by heat. They can be converted into toxoids that are used as vaccines. Exotoxins are extrememly powerful biological poisons as in the case of tetanus. It has been estimated that 1mg of purified tetanus toxin could kill millions of mice. Synthetic peptide corresponding to aa1300-1314 of Clostridium tetani tetanus toxin, CDWYFVPTDEGWTND. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
