Thioredoxin Reductase, Recombinant, E. coli (TRXR, trxB)

Produktübersicht

• Artikelname: Thioredoxin Reductase, Recombinant, E. coli (TRXR, trxB)
• Artikelnummer: USB-T5010-14E
• Hersteller Artikelnummer: T5010-14E
• Alternativnummer: USB-T5010-14E-20,USB-T5010-14E-100,USB-T5010-14E-500
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: WB

Produktbeschreibung

TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant enzyme corresponding to aa1-321 of human TRXB expressed in E. coli. Amino Acid Sequence: MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K Enzyme Activity: Specific activity is >10 units/mg, measured in a coupled assay with DTNB and NADPH. The amount of TNB generated was determined by measuring absorbance at 412nm. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 34.6
• NCBI: 003044110
• UniProt: P0A9P4
• Reinheit: 90% (SDS-PAGE). Purified by using conventional chromatography techniques.
• Formulierung: Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.