Synthetic peptide corresponding to C-PKSTYESNTKQ, from human TIA1, at the internal region of the protein (NP_071320.1, NP_071505.1). Species sequence homology: canine, mouse and rat
The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15kD protein that is thought to be derived from the carboxyl terminus of the 40kD product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:128,000 Western Blot: 0.1-0.3ug/ml, observed in lysates of cell line Jurkat on ~40kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
