Tissue cDNA, First Strand, Human Adult Disease, Lung, Emphysema, BioGenomics(TM)

Produktübersicht

• Artikelname: Tissue cDNA, First Strand, Human Adult Disease, Lung, Emphysema, BioGenomics(TM)
• Artikelnummer: USB-T5595-0142A
• Hersteller Artikelnummer: T5595-0142A
• Alternativnummer: USB-T5595-0142A-40
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

BioGenomics(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection. PCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA, and it can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetal normal tissues. Features: Ready to use for PCR Oligo dT primer used to ensure the entire 3 end of cDNA is present With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA The largest selection of cDNAs from different tissues on the market Documentation of tissues clinical histories available (additional cost) Applications: Immediate PCR Amplification of known genes Verification of genetic mutation Comparison of a specific gene between different tissues Analysis of mRNA alternative splicing Gene cloning and target sequencing Contents: 40ul or 10ul per package Specifications: cDNA Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 1ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT Reaction stopped by heating at 65C for 10 minutes. The cDNA is in 1X RT buffer. (1X RT Buffer: 50mM Tris-CI, pH 8.3, 75mM KCI, 3mM MgCI2, 10mM DTT). The estimated cDNA concentration is about 2.5 ng/ul. 1ul cDNA is good enough for one PCR reaction. The cDNA panel is comprised of 5 cDNAs from 5 different tissues. Each of them contains 10ul of cDNA. Human cDNA matched pair. Products include: Primary Pair (PP), or Primary and Metastatic Pair (PM). PP consists of cDNAs isolated from primary tumor (or diseased tissue) and its adjacent normal tissue, PM consists of cDNAs from primary tumor and corresponding metastatic tumor. cDNAs in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific (or disease specific) genes and tumor metastatic genes. Universal cDNA is prepared by reverse transcribed Universal RNA by using random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes Human Universal cDNA is prepared from major organs of both male and female adult donors Dog (Beagle) Universal cDNA is prepared from major organs of both male and female adult donors Monkey (Cynomolgus and Rhesus) Universal cDNA is prepared from major organs of both male and female adult donors Mouse Universal cDNA is prepared from several male and female Balb/C mice whole bodies without fur Rat Universal cDNA is prepared from several male and female Sprague Dawley or Wistar rats whole bodies without fur. 1ul cDNA is good for one PCR reaction. The 5 end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs. Storage Conditions: Store cDNAs at -20C Unique Features of cDNA: PCR Ready First Strand cDNA is made from our high-quality total RNA. Each cDNA is a premade, tissue-specific ''pool of first strand cDNA from which full-length genes can be amplified by using sets of gene-specific primers. They can also be used to study tissue-specific gene expression and to find not only polymorphic forms of mRNA but also mRNA belonging to a multigene family. The following features have been integrated into our cDNA products to ensure their superior quality. 1. Three formats of cDNA products are available There are three types of cDNA products to choose from: PCR ready first strand cDNA, tumor/adjacent normal paired cDNA,