Tissue cDNA, First Strand, Human Tumor, Colon, BioGenomics(TM)

Produktübersicht

• Artikelname: Tissue cDNA, First Strand, Human Tumor, Colon, BioGenomics(TM)
• Artikelnummer: USB-T5595-0459
• Hersteller Artikelnummer: T5595-0459
• Alternativnummer: USB-T5595-0459-40
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

BioGenomics(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection. PCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA, and it can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT Reaction stopped by heating at 65C for 10 minutes. The cDNA is in 1X RT buffer. (1X RT Buffer: 50 mM Tris-Cl, pH 8.3, 75mM KCl, 3mM MgCI2, 10mM DTT). 1ul cDNA is good for one PCR reaction. The 5 end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs. Features: Ready to use for PCR Oligo dT primer used to ensure the entire 3 end of cDNA is present With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA The largest selection of cDNAs from different tissues on the market Documentation of tissues clinical histories available (additional cost) Applications: Immediate PCR Amplification of known genes Verification of genetic mutation Comparison of a specific gene between different tissues Analysis of mRNA alternative splicing Gene cloning and target sequencing Contents: 40ul or 10ul per package Storage Conditions: Store cDNAs at -20C Unique Features of cDNA: PCR Ready First Strand cDNA is made from our high-quality total RNA. Each cDNA is a premade, tissue-specific ''pool of first strand cDNA from which full-length genes can be amplified by using sets of gene-specific primers. They can also be used to study tissue-specific gene expression and to find not only polymorphic forms of mRNA but also mRNA belonging to a multigene family. The following features have been integrated into our cDNA products to ensure their superior quality. 1. Three formats of cDNA products are available There are three types of cDNA products to choose from: PCR ready first strand cDNA, tumor/adjacent normal paired cDNA, and cDNA panels. 2. RNAs with high quality are used for cDNA synthesis The cDNA synthesis will not be successful using degraded or contaminated RNA, because degraded RNA will not generate full-length cDNA, and contamination may inhibit the synthesis of cDNA. The RNAs isolated by BioChains proprietary technology are pure and intact from primary tissues. These RNAs therefore ensure a very high quality of cDNAs. 3. Efficient reverse transcription is guaranteed An incorporation test is integrated into our quality control procedure for cDNA synthesis. Every cDNA product must pass a standard high incorporation. 4. cDNA is full length All cDNA products are reverse transcribed with oligo dT primer, and 5 selected. With the cDNAs as template, 5 and 3 regions of Clathrin, a relatively low abundance gene, are amplified by PCR. The generation of PCR products indicates the gene is not only expressed but is also full length. Since the size of clathrin is over 6kb, any genes less than 6kb are guaranteed to be full length. 5. The cDNA gene expression profile is the same as the mRNA profile The expression patterns of any genes from different tissue cDNAs are the same as total RNA or mRNA. The cDNA can be used not only for amplification of a gene fragment but also for gene quantification. 6. Placenta cDNA is established as a gene expression standard Any gene expression data generated anywhere or anytime can be compared with this standard.