TLR1 Fc Chimera, Recombinant, Human (Toll-like receptor 1, CD281, KIAA0012, rsc786, TIL, Toll/interleukin-1 receptor-like protein)
Artikelnummer:
USB-T8050-04B
Hersteller Artikelnummer:
T8050-04B
Alternativnummer:
USB-T8050-04B-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
The Tolllike family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2) . TLRs contain a large number of leucinerich repeats (LRRs) and a cytoplasmic tail with one Tol l /IL1 receptor (TIR) domain. Mature human TLR1 consists of a 556 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (3, 4). Within the ECD, human TLR1 shares 63% aa sequence identity with human TLR6 and 20% 43% aa sequence identitity with human TLR2, 3 , 4 , 5 , 7 , 8 , 9, and 10. It shares 73% and 71% aa sequence identity with mouse and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligandindependent association with TLR2 (5 8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (911). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosyl phosphatidylinositols (6,1012). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of N Fkappa B (7,11,13). Description: NSO-expressed recombinant protein corresponding to human TLR1(Ser22 Asn578, AccessionAAC34137) linked using the sequence SIEGRMD to human IgG1 (Pro100 Lys330). Structure: Disulfide linked homodimer N-terminal Sequence Analysis: Ser22 Molecular Mass: Predicted: 89.9kD Observed: 95-105kD, (SDS-PAGE, reducing conditions) Activity: Measured by its ability to bind biotinylated Pam3CysSerLys4 (PAM3CSK4).
