TLR2 (Toll Like Receptor 2) (FITC), Clone: [2B4A1], Mouse, Monoclonal
Artikelnummer:
USB-T8050-12K
Hersteller Artikelnummer:
T8050-12K
Alternativnummer:
USB-T8050-12K-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
FC
Immunogen:
Recombinant human TLR2.
The Toll-like receptor (TLR) family is comprised of Type I transmembrane proteins characterized by an extracellular leucine-rich domain and a cytoplasmic tail that contains a conserved region call the Toll/IL-1 (TIR) domain. First discovered in Drosophila, TLRs recognize specific molecular patterns that are present in microbial components and respond to these components in order eliminate or limit invading microbes. To date, ten mammalian homologs of TLRs have been described. Activation of TLR2 leads to the initiation of apoptosis and the activation of the NK-kappaB pathway by the adaptor molecule MyD88. TLR2 recognizes and is activated by components from a broad variety of microorganisms including lipoproteins from Gram-negative bacteria, Mycoplasma, spirochetes, peptidoglycan and LTAs from Gram-positive bacteria, lipoarabinomannan from mycobacteria, glycoinositolphospholipids, phenol-soluble modulin, zymosan from yeast and neisserial porins. TLR2 has been reported to functionally associate with TLR1 and TLR6 in order to recognize various lipopeptides. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
