Synthetic TLR5 peptide of 15aa near the carboxy terminus of human TLR5 (GenBank accession no. NP_003259).
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-kB, leading to the activation of TNF-a and other cytokines (4). A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires disease and demonstrates the importance of TLR5 in the innate immune response (5). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Blocking Peptide: T8050-43B1: TLR5, CT, Control Peptide (Toll Like Receptor 5, Toll/Interluekin-1 Receptor-Like Gene 3, TIL3) Positive Control: THP-1 cell lysate Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
