Torsin A (Torsin family 1 member A, Dystonia 1 protein), IgG1, Clone: [7i57], Mouse, Monoclonal
Artikelnummer:
USB-T8067-37
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| Artikelname: | Torsin A (Torsin family 1 member A, Dystonia 1 protein), IgG1, Clone: [7i57], Mouse, Monoclonal |
| Artikelnummer: | USB-T8067-37 |
| Hersteller Artikelnummer: | T8067-37 |
| Alternativnummer: | USB-T8067-37-100 |
| Hersteller: | US Biological |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | IP, WB |
| Immunogen: | Maltose binding protein fusion protein from human torsin-A |
| TorsinA is an AAA(+) protein that has been demonstrated as an endoplasmic reticulum (ER) chaperone protein involved in a sensitive reporter system for quantitation of processing through the secretory pathway. It is predominantly located in the lumen of the ER and nuclear envelope. TorsinA is responsible for early onset torsion dystonia (DYT1 dystonia), a dominantly inherited movement disorder and disease of basal ganglia function. DYT1 dystonia is commonly caused by the deletion of a glutamic acid (DeltaE) in the carboxyl terminal region of TorsinA, where the protein then aggregates in perinuclear inclusions instead of the ER. TorsinA is degraded primarily through the macroautophagy-lysosome pathway, whereas the TorsinA DeltaE mutant protein is degraded by both the proteasome and macroautophagy-lysosome pathways. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:100 on 4% paraformaldehyde fixed (37C for 30 minutes) cells. Suggested permeabilization method is 0.1% NP40 for 20 minutes. Immunoprecipitation: The preferred tissue/cell lysis buffer is 1% NP40 and/or RIPA. Recommended antibody amount is 3-4ul of ascites. Final reaction volume of 1000ul. Final total protein concentration in reaction mix: 0.1-0.5mg/ml. Suggested incubation time is 16 hours. Sugggested capture is Protein A/G. Western Blot: 1:200. The antibody reacts with the ~37kD torsin A protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 0.05% Tween-20. Optimal dilutions to be determined by the researcher. Recommended Positive Control: Human brain lysate, NGF treated PC12 cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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