TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

Produktübersicht

• Artikelname: TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)
• Artikelnummer: USB-T8180
• Hersteller Artikelnummer: T8180
• Alternativnummer: USB-T8180-10,USB-T8180-50
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Human TRAIL (TNF-Related Apoptosis Inducing Ligand, Apo2 Ligand), a 19.6kD protein consisting of 169 amino acid residues, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. Biological Activity: Assay 1: Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml. Assay 2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 0.8-2.0 ng/ml. Amino Acid Sequence: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 19.6
• NCBI: 003801
• UniProt: P50591
• Reinheit: 98% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug (1EU/µg)
• Formulierung: Supplied as a lyophilized powder from PBS. No preservative added. Reconstitute with sterile PBS to a concentration of 1mg/ml.