Activating enhancer-binding protein 2-beta (AP-2 beta, TFAP2B), Goat
Artikelnummer:
USB-T8195-52E3
Hersteller Artikelnummer:
T8195-52E3
Alternativnummer:
USB-T8195-52E3-100
Hersteller:
US Biological
Wirt:
Goat
Kategorie:
Antikörper
Applikation:
WB
Immunogen:
Recombinant protein corresponding to Leu121-Phe218 from human AP-2 beta, expressed in E. coli.
AP-2 beta (Activating enhancer-binding protein 2-beta, also TFAP2B) is a 52kD member of the AP-2 family of transcription factors. It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme. In adults, AP-2 beta is expressed in renal epithelium. Human AP-2 beta is 460aa in length. It contains a transactivation domain (aa41-131), a DNA-binding motif (aa132-298) and a C-terminal dimerization region (aa299-439). SUMOylation occurs on Lys21, adding 15kD of MW in SDS-PAGE. AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma. Dimers appear important for facial and limb development. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using lysates of SH-SY5Y human neuroblastoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
