TFEC (Transcription Factor EC, TFE-C, Class E Basic Helix-loop-helix Protein 34, bHLHe34, Transcription Factor EC-like, hTFEC-L, BHLHE34, TCFEC, TFECL), Goat
Artikelnummer:
USB-T8196-50D1
Hersteller Artikelnummer:
T8196-50D1
Alternativnummer:
USB-T8196-50D1-100
Hersteller:
US Biological
Wirt:
Goat
Kategorie:
Antikörper
Applikation:
ELISA, WB
Immunogen:
Synthetic peptide corresponding to TLDHQIINPTLK-C, from human TFEC, at N-terminal (NP_036384.1, NP_001018068.1).
Expression of the mouse transcription factor EC (Tfec) is restricted to the myeloid compartment, suggesting a function for Tfec in the development or function of these cells. However, mice lacking Tfec develop normally, indicating a redundant role for Tfec in myeloid cell development. It is reported that Tfec is specifically induced in bone marrow-derived macrophages upon stimulation with the Th2 cytokines, IL-4 and IL-13, or LPS. LPS induced a rapid and transient up-regulation of Tfec mRNA expression and promoter activity, which was dependent on a functional NF-kappaB site. IL-4, however, induced a rapid, but long-lasting, increase in Tfec mRNA, which, in contrast to LPS stimulation, also resulted in detectable levels of Tfec protein. IL-4-induced transcription of Tfec was absent in macrophages lacking Stat6, and its promoter depended on two functional Stat6-binding sites. A global comparison of IL-4-induced genes in both wild-type and Tfec mutant macrophages revealed a surprisingly mild phenotype with only a few genes affected by Tfec deficiency. These included the G-CSFR (Csf3r) gene that was strongly up-regulated by IL-4 in wild-type macrophages and, to a lesser extent, in Tfec mutant macrophages. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.5-2ug/ml, human testis lysate observed on ~40-45kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
