Recombinant protein corresponding to human transferrin receptor
The human transferrin receptor (TfR) is a homodimeric type II membrane protein consisting of two identical 95kD subunits covalently linked by intermolecular disulfide bonds. The TfR has an extracellular domain of 671 amino acids, a single 28 residue transmembrane region and a 61 residue amino terminal cytoplasmic domain. Expression of the receptor is limited on normal tissue, however, highly expressed on the surface of some tumors. TfR is included in the class of ligand transport receptors which are internalized and clustered in coated pits. An important function of the receptor is to bind the serum transport protein transferrin (Tf) and mediate the uptake of iron into the cell. A growing area of research involves the use of monoclonal anti-TF receptor antibodies to inhibit the uptake of iron and thus block tumor cell growth. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence/ Immunocytochemistry and Electron Microscopy. Other applications have not been tested. Recommended Dilutions: Immunofluorescence (IC): 1:250 Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[6A202]
Isotyp:
IgG1
Reinheit:
Purified by affinity chromatography
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
