Recombinant protein corresponding to Val40-Ala89 from human TGF alpha, expressed in E. coli.
TGF-alpha (transforming growth factor alpha) is a 50aa EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS). TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis. Applications: Suitable for use in Direct ELISA, Immunohistochemistry, Neutralization and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin embedded sections of human astrocytoma, pituitary adenoma, and prostate cancer tissue subjected to Antigen Retrieval Reagent-Basic. Neutralization: 0.4-0.8ug/ml in the presence of 3ng/ml recombinant human TGF-a. Measured by its ability to neutralize TGF-a-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
