TGF-alpha is a 50aa EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS). TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and RIA. Other applications not tested. Recommended Dilutions: ELISA: 1:16,000 Immunohistochemistry: 1:25-1:100 Immunoprecipitation: 1:250-1:1000 RIA: 1:2,000-1:100,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
