Recombinant protein corresponding to Leu30-Arg 278 (Cys33Ser) from human TGF-beta 1, expressed in S. frugiperda insect ovarian cell line Sf 21.
TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta associated non-covalently with a protein designated the latency associated peptide (LAP). TGf-beta and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta inactive and inaccessible to anti-TGF-beta antibodies. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 human platelets Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
