Recombinant human TGF-beta1, expressed in HEK293 cells.
Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Indirect): 4-8ug/ml detects at least 100pg/ml of rcombinant human TGF-beta 1 using 100ul/well. Western Blot: 2-4ug/ml. The detection limitis 0.25-0.5ng/lane under reducing or non-reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.