Transforming Growth Factor beta Receptor III (Transforming Growth Factor beta Receptor Type 3, TGF-beta Receptor Type 3, TGF-beta Receptor Type III, TGFBR3, TGFR-3, Betaglycan, BGCAN), Clone: [11C1084D11G10], Rabbit, Monoclonal
Artikelnummer:
USB-T8250-50
Hersteller Artikelnummer:
T8250-50
Alternativnummer:
USB-T8250-50-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IP, WB
Immunogen:
Synthetic peptide corresponding to residues in the extracellular domain of human TGF-b receptor III
Transforming growth factor-b (TGF-b) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis. TGF-b elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases. The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-b with high affinity but lacks a cytoplasmic signaling domain. Expression of the type III receptor can regulate TGF-b signaling through presentation of the ligand to the signaling complex. The only known direct TGF-b signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
