Transforming Growth Factor beta2, Recombinant, Human (TGFb2, Polyergin, Cetermin, G-TSF, Glioblastoma-derived T-cell suppressor factor, BSC-1 cell growth inhibitor)
Artikelnummer:
USB-T8251-01
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| Artikelname: | Transforming Growth Factor beta2, Recombinant, Human (TGFb2, Polyergin, Cetermin, G-TSF, Glioblastoma-derived T-cell suppressor factor, BSC-1 cell growth inhibitor) |
| Artikelnummer: | USB-T8251-01 |
| Hersteller Artikelnummer: | T8251-01 |
| Alternativnummer: | USB-T8251-01-10 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| TGF-B is a multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGF-B is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. It is a family of regulatory proteins consisting of a number of proteins distantly related. TGF-B1 has been found in the highest concentration in human platelets and mammalian bone, but is produced by many cell types in smaller amounts. TGF-B2 has been found in the highest concentration in porcine platelets and mammalian bone, but again is also produced by many types of cells. The heterodimer, TGF-B1.2, has so far been found only in small amounts in porcine platelets. TGF-B3 has been detected in human, porcine, and avian sources, mainly in cells of mesenchymal origin, suggesting a different role for this protein than for TGF-B1 or TGF-B2. TGF-B4 has been detected so far only in chick embryo chondrocytes, and its distribution in other types of cells has not yet been characterized. TGF-B5 has been detected only in Xenopus embryos. TGF-B1, TGF-B2, and TGF-B1.2 appear to be largely equivalent in biological activity, although there appear to be differences in binding to certain types of receptors, and there are a few reports of differential responses to TGF-B1 and TGF-B2. Recombinant Human TGF-beta2 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond, expressed in (BTI-Tn-5B1-4) Hi-5 Insect cells. Molecular Weight: ~25kD Biological Activity: Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED50 was found to be 0.2 ng/ml, corresponding to a specific activity of 5 x 10e6 units/mg. Amino Acid Sequence (monomer): ALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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