| Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer. One of the biological buffers. Synonyms: Tris(hydroxymethyl)aminomethane HCl, 2-Amino-2-(hydroxymethyl)-1,3-propanediol HCl, Tromethamine HCl CAS No: C4H11NO3HCl Molecular Formula: C4H11NO3HCl Molecular Weight: 157.60 Purity: 99% Appearance: White, crystalline powder pH (1.0M, H2O): 3.5-5.0 pKa (20C:) 8.3 0.15 Absorbance (260nm): 0.01 Absorbance (280nm): 0.05 Solubility (1M, H2O): Colorless, clear, complete Loss on Drying: 0.3% Residue on Ignition: 0.1% Melting Point: 150-154C Heavy Metals (Pb): 0.0005% Iron (Fe): 0.005% Insoluble Matter: <0.005% RNase: None Detected DNase: None Detected Protease: None Detected Storage: RT |
