NS0-derived, recombinant human TROP-2 extracellular domain, His27-Thr274.
TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas. Cell surface expression of TROP-2 is determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515-545 nm. Applications: Suitable for Flow Cytometry. Designed to quantitatively determine the percentage of cells bearing TROP-2 within a population and qualitatively determine the density of TROP-2 on cell surfaces by flow cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul is added to1-2.5x10e5 cells. Optimal dilutions to be determined by the researcher. Storage: Lyophilized samples are stable for twelve months from date of receipt when stored at -20C to -70C. Upon reconstitution, the antibody can be stored at 2-8C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquotted and stored frozen at -20C to -70C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
