Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) (HRP), IgG1, Clone: [7E143], Mouse, Monoclonal

Produktübersicht

• Artikelname: Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) (HRP), IgG1, Clone: [7E143], Mouse, Monoclonal
• Artikelnummer: USB-T8665-16E-HRP
• Hersteller Artikelnummer: T8665-16E-HRP
• Alternativnummer: USB-T8665-16E-HRP-100
• Hersteller: US Biological
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: Human troponin complex

Produktbeschreibung

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place Because of which myosin can not bind the actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confirmational changes which leads to dislocation of troponin I and finally tropomyosin leaves the the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character. Applications: Suitable for use in Western Blot and Sandwich ELISA. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells of Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [7E143]
• Isotyp: IgG1
• Reinheit: Purified by Protein A affinity chromatography from ascites.
• Formulierung: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Horseradish peroxidase (HRP).