Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) (PE), IgG1, Clone: [14e1], Mouse, Monoclonal

Produktübersicht

• Artikelname: Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3) (PE), IgG1, Clone: [14e1], Mouse, Monoclonal
• Artikelnummer: USB-T8665-20B-PE
• Hersteller Artikelnummer: T8665-20B-PE
• Alternativnummer: USB-T8665-20B-PE-100
• Hersteller: US Biological
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FLISA, WB
• Immunogen: Human Cardiac Troponin I (cTnI) (24-40 region)

Produktbeschreibung

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter ''I is given due to its inhibitory character. Tissue-specific Troponin Subtypes: Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) Cardiac troponin I, TNNI3 (19q13.4, 191044) Applications: Suitable for use in FLISA., Immunoaffinity Purification, and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Matched Pair: Suitable for use as the detection or conjugate (label) antibody. Matched to T8665-20A as the capture or solid phase antibody. Hybridoma: Sp2/0 myeloma cells with spleen cells of Balb/c mice Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [14e1]
• Isotyp: IgG1
• Reinheit: Purified by Protein A affinity chromatography from ascites.
• Formulierung: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).