Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I and to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure. Applications: Suitable for use in ELISA, Immunoaffinity Purification and Western Blot. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Recommended Pair: Suitable for use as the capture or solid phase antibody. Matched to T8665-22A, T8665-22B or T8665-30A as the detection or conjugate (label) antibody. Hybridoma: Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
