Tru1I (MseI)

Produktübersicht

• Artikelname: Tru1I (MseI)
• Artikelnummer: USB-T8666-50
• Hersteller Artikelnummer: T8666-50
• Alternativnummer: USB-T8666-50-300,USB-T8666-50-1500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-T T A A-3 3-A A T T-5 Concentration: 10u/ul Source: HC Thermus ruber RFL1 Buffer: 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 65C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Enzyme Properties: Methylation Effects: Dam: never overlaps - no effect. Dcm: never overlaps - no effect. CpG: never overlaps - no effect. EcoKl: may overlap - blocked. EcoBl: never overlaps - no effect. Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Only small amounts of enzyme (up to 20 units) can be inactivated by incubation at 80C in 20 minutes. Compatible Ends: Csp6I, MaeI, NdeI, VspI Number of Recognition Sites in DNA: Lambda: 195 PhiX174: 35 M13mp18/19: 63 pBR322: 15 pUC18/19: 13 pUC57: 13 pTZ19R/U: 18 Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Tru1I. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Tru1I, more than 90% of the DNA fragments can be ligated at a 5-termini concentration of 1.2uM. More than 90% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.