Tryptophan Hydroxylase, Tyrosine Hydroxylase & Phenylalanine Hydroxylase (TH, TYH, TRH, TYH, PAH, PH8, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase) (HRP), IgG1, Clone: [2Q122 PH8], Mouse, Monoclonal
Artikelnummer:
USB-T9014-70A
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| Artikelname: | Tryptophan Hydroxylase, Tyrosine Hydroxylase & Phenylalanine Hydroxylase (TH, TYH, TRH, TYH, PAH, PH8, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase) (HRP), IgG1, Clone: [2Q122 PH8], Mouse, Monoclonal |
| Artikelnummer: | USB-T9014-70A |
| Hersteller Artikelnummer: | T9014-70A |
| Alternativnummer: | USB-T9014-70A-100 |
| Hersteller: | US Biological |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | IHC, IP, WB |
| Immunogen: | Human phenylalanine hydroxylase sequence between aa139-155. |
| Tyrosine hydroxylase (TYH) is the enzyme which converts tyrosine to dihydroxyphenylalanine (L-dopa), a precursor of the catecholamine neurotransmitters dopamine, noradrenaline and adrenaline. Tryptophan hydroxylase (TRH) is the enzyme that converts 5-hydroxytryptophan to serotonin. In fresh tissue, the antibody binds to TYH and TRH. So it is a marker for TYH-containing catecholaminergic neurons and serotonergic neurons. Tryptophan hydroxylase (TRH) can be used as a marker for serotonin as it converts 5-hydroxytryptophan to serotonin. Serotonin is rapidly metabolized and is unable to be detected by anti-serotonin antibodies in post-mortem tissue. In human tissue that has been formalin-fixed, due to a change in the antigenic determinant of tyrosine hydroxylase (TYH), the antibody binds only tryptophan hydroxylase (TRH). Used to specifically identify serotonergic neurons in fixed human tissue. Phenylalanine hydroxylase (PAH) can also be detected in hepatic tissue sections. Applications: Suitable for use in the identification of dopaminergic and serotonergic neurons by Immunohistochemistry. Also suitable for Western Blot, Immunoprecipitation and Immunohistochemistry of TYH, PAH and TRH. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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