| Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications have not been tested. Recommended Dilutions: ELISA: 1:10,000-1:50,000 using 50-100ng control peptide per well. Western Blot: 1-10ug/ml using ECL. Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
