Synthetic peptide corresponding to an epitope within the C-terminal region, ESESQGPK, aa441-448, of human class III beta tubulin.
Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of beta III is almost exclusively neuron-specific. Applications: Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen sections, paraffin sections): 5-20ug/ml. Pretreatment with 0.1% pepsin (trypsin) in 0.1M HCl, Incubate for 30 min at RT or high temperature citrate buffer antigen retrieval. Western Blot: 1-10ug/ml Optimal dilutions to be determined by the researcher. Recommended Secondary Reagents: F(ab)2 rabbit anti-mouse IgG (HRP) Recommended Negative Controls: Mouse IgG1 Negative Control Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
