C-terminal peptide of gamma-tubulin counjugated to KLH.
The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1-2ug/ml. Cells must be fixed prior to staining. Fix cells for 10 min in either methanol at -20C and for 6 min in acetone at -20C, or in methanol alone for 10 min at -20C, or in acetone for 10 min at -20C. After fixing cells, incubate for 45 minutes at RT. Western Blot: 1-2ug/ml, reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
