Three synthetic peptides corresponding to different regions of the beta-Tubulin 3, conjugated to KLH. Species Sequence Homology: human and rat, 100%.
Human beta-Tubulin 3 is a 50.432kD structural protein (450aa) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:2000-1:5000 Immunohistochemistry/Immunocytochemistry: 1:1000-1:2000 detects Tubulin, beta 3 in 2% paraformaldehyde-fixed tissues or cells. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in 10mM PBS, pH 7.2, 0.02% sodium azide.
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