Tumor Necrosis Factor alpha (TNFa) (Biotin), Clone: [MP6-XT3], Rat, Monoclonal
Artikelnummer:
USB-T9160-16B
Hersteller Artikelnummer:
T9160-16B
Alternativnummer:
USB-T9160-16B-500
Hersteller:
US Biological
Wirt:
Rat
Kategorie:
Antikörper
Applikation:
ELISA, WB
Immunogen:
Recombinant protein corresponding to mouse TNF-alpha, expressed in E. coli.
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying mouse TNFa protein levels. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100ng/lane of recombinant mouse TNFa under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
