Tumor Necrosis Factor Receptor Type 2 (TNF-R2, CD120b, p75/p80, Tumor necrosis factor receptor superfamily member 1B precursor, Tumor necrosis factor receptor type II) (FITC), IgG1, Clone: [9G297 80M2], Mouse, Monoclonal

Produktübersicht

• Artikelname: Tumor Necrosis Factor Receptor Type 2 (TNF-R2, CD120b, p75/p80, Tumor necrosis factor receptor superfamily member 1B precursor, Tumor necrosis factor receptor type II) (FITC), IgG1, Clone: [9G297 80M2], Mouse, Monoclonal
• Artikelnummer: USB-T9161-20F
• Hersteller Artikelnummer: T9161-20F
• Alternativnummer: USB-T9161-20F-100
• Hersteller: US Biological
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, FLISA, IF, IP

Produktbeschreibung

TNF-R2 is present on most cell types and is considered to be an important co-stimulator together with TNF-R1 in cellular responses to TNF-alpha, in particular the transmembrane form of TNF-alpha. A dominant role of TNF-R2 has been shown in thymocyte activation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-R1 and TNF-R2, which have both distinct and overlapping downstream signalling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-R1. TNF-R1 is equally well activated by both the 17kD soluble and 26kD membrane-bound form, whereas TNF-R2 is activated only by the membrane bound form of TNF-alpha. T9161-20F is a non-agonistic receptor modulating antibody. It enhances in vitro TNF alpha responses by increasing the affinity of the soluble form of TNF-alpha for TNF-R2. Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Immunoprecipitation and Functional Studies. Other applications not tested. Recommended Dilution: Immunofluorescence/Immunocytochemistry (2): 1:50 Flow Cytometry (2): 1:50 Functional Studies (1,2,4,5): functions as an allosteric modulator, which stabilizes the ligand-receptor complex. Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells transfected with TNF-RII Negative Control: Mock transfected HeLa cells Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [9G297 80M2]
• Isotyp: IgG1
• Reinheit: Purified by Protein G affinity chromatography.
• Formulierung: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).