TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5ug/ml using 100ul/well antibody solution detects 0.2-0.4ng/well of recombinant human TWEAK. Western Blot: 0.1-0.2ug/ml detects 1.5-3.0ng/lane, under either reducing or or non-reducing conditions. Optimal dilutions to be determined by researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Reinheit:
Purified by affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
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