Since the original observations that protein tyrosine kinases (PTKs) play critical roles in oncogenic transformation (1,2), it has become clear that both protein tyrosine kinases and phospho-tyrosine phosphatases control a wide range of critical biological processes (3,4). Antibodies specific for phospho-tyrosine (5,6) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies provide exceptionally sensitive new tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Applications: Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:2000 Immunoprecipitation: 1:50 Immunohistochemistry (paraffin): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[3A43P-Tyr-100]
Isotyp:
IgG1
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Labeled with Biotin.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
