KLH conjugated synthetic phosphopeptide from rat Tyrosine Hydroxylase, phosphorylated (Ser31). Species Sequence Homology: primate
Tyrosine hydroxylase (TH) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines dopamine and norepinephrine from tyrosine. TH is therefore a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. Phosphorylation of Ser31 can activate TH in vitro, and has been observed at this residue following NGF, phorbol ester, and diacylglycerol treatment of PC12 cells. Ser31 has been identified as a membrane depolarization-dependent and growth factor-sensitive phosphorylation site. Applications: Suitable for use in Western Blot, Immunocytochemistry, and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunocytochemistry: 1:50-1:300 Immunohistochemistry: 1:300-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
