TWIST2 (Twist-related Protein 2, Class A Basic Helix-loop-helix Protein 39, bHLHa39, Dermis-expressed Protein 1, Dermo-1, BHLHA39, DERMO1), Rabbit
Artikelnummer:
USB-T9300-08A
Hersteller Artikelnummer:
T9300-08A
Alternativnummer:
USB-T9300-08A-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA
Immunogen:
Synthetic peptide corresponding to 20aa mouse Twist2, N-terminal (KLH).
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by Twist gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype. Twist and Dermo1, also called Twist1 and Twist2 respectively, were induced by a cytokine signaling pathway that required the dorsal-related protein Rela, a member of the nuclear factor kappa-B family of transcription factors, in mice. Twist1 and Twist2 repressed cytokine gene expression through interaction with Rela. TWIST proteins regulate cytokine signaling by establishing a negative feedback loop that represses the NFKB-dependent cytokine pathway. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:100,000 (50-100ng control peptide/well) Western Blot: 1-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
