Synthetic peptide corresponding to C-HLRDKEDFRNKVDD, from the C-Terminus of human UBE2F (NP_542409.1).
UBE2F (Ubiquitin-conjugating enzyme E2F), a ubiquitously expressed enzyme and member of the ubiquitin-like E2 conjugating enzyme family, which acts as a specific NEDD8 conjugating enzyme, by accepting NEDD8 from the Uba3-NAE1 (APP-BP1/Uba3) E1 complex. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:16,000 Western Blot: 0.05-0.2ug/ml detects ~20kD in Mouse Liver lysates (calculated MW of 21.1kD according to Human NP_542409.1 and to Mouse NP_080730.1). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
