Humanized Di-ubiquitin linked at K63. Epitope: Lys63 ubiquitination site
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFkappaB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Inhibition. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation: Precipitated ubiquitin-containing proteins from HeLa cell lysates. Immunohistochemistry: Ubiquitin (Lsy63 specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Immunocytochemistry: Ubiquitin (Lsy63 specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat and rabbit brain sections. Inhibition: Preincubation of U1000-06Q with K63- multiubiquitin chains inhibited binding to HeLa cell lysate in Western Blots. Optimal dilutions to be determined by the researcher. Positive Control: Lys63 polyubiquitin chains Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
