UBE2D1, CT (Ubiquitin-conjugating Enzyme E2-D1, Ubiquitin-protein Ligase D1, Ubiquitin Carrier Protein D1, UbcH5, Ubiquitin-conjugating Enzyme E2-17kD 1, Ubiquitin-conjugating Enzyme E2(17)KB 1, UBC4/5 Homolog, Stimulator of Fe Tr

Produktübersicht

• Artikelname: UBE2D1, CT (Ubiquitin-conjugating Enzyme E2-D1, Ubiquitin-protein Ligase D1, Ubiquitin Carrier Protein D1, UbcH5, Ubiquitin-conjugating Enzyme E2-17kD 1, Ubiquitin-conjugating Enzyme E2(17)KB 1, UBC4/5 Homolog, Stimulator of Fe Tr
• Artikelnummer: USB-U1000-86M
• Hersteller Artikelnummer: U1000-86M
• Alternativnummer: USB-U1000-86M-200
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC, WB
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa111-140 in the C-terminal region of human UBE2D1. Species sequence homology: bovine, rat

Produktbeschreibung

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Reinheit: Purified by ammonium sulfate precipitation
• Formulierung: Supplied as a liquid in PBS, 0.09% sodium azide.